lncRNA F11-AS1 Suppresses Glioma by Regulation miRNA-3146/PTEN

Abstract BACKGROUND: LncRNAs play key roles in glioma development, however, lncRNA F11-AS1’s effects has been unclearly in glioma.OBJECTIVE: F11-AS1 is an anti-tumor factor in glioma has been clearly until now.METHODS: Evaluating F11-AS1 expression by ISH in difference tissues and analysis the correlation with glioma prognosis in clinical. Using U87 and U251 cell to discuss lncRNA F11-AS1’s effect in glioma cancer cell biological activities by MTT, flow cytometry, transwell and wound healing assay. And measuring the relative miRNA and gene expressions by RT-qPCR and relative proteins expressions by WB assay. In next step, discuss the miR-3146 in F11-AS1 depressing cancer cell biological activities.RESULTS: The authors observed in this study that F11-AS1 was down-regulation in glioma and negatively correlated with miRNA-3146. Servival analysis showed that low level of F11-AS1 predicted poor survival. In glioma cells, F11-AS1 knockdown led to up-regulate, whereas F11-AS1 overexpression led to down-regulated miRNA-3146. Analysis of cell biological activities showed that F11-AS1 overexpression had effects to suppress glioma cell lines biological activities.CONCLUSION: Therefore, F11-AS1 down-regulates miRNA-3146 to depress cell biological activities in glioma.