Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Modern methods to determine vitamin E in foods and feeds
View through CrossRef
Highly specific and sensitive, the proposed fluorometric method for determining α-tocopherol is robust and fairly fast. Ithas been tested in parallel with a conventional thin layer chromatographic method on foods and feeds. The only necessarycleanup is the usual saponification. The unsaponificable fraction can be extracted with ethyl ether or, preferably, withExtrelut columns. Reagents and their solvents are added to the isooctane solution before each successive reaction and arethen eliminated by partition with water. The α-tocopherol (α-T) derivative always remains in isooctane. The first step isnitrosation and elimination of tocopherols and tocotrienols other than α-isomers. α-T is then oxidized to α-tocored (α-TR)with a mixture of sulfuric acid, ferric chloride, and iodine bromide. Α-TR is then condensed to a new reagent: 4,5-dimethylo-phenylenediamine. The phenazine formed is strongly fluorescent. Iodine and bromine add to the double bonds of α-tocotrienol present and quench the fluorescence of its phenazine.
University of Debrecen/ Debreceni Egyetem
Title: Modern methods to determine vitamin E in foods and feeds
Description:
Highly specific and sensitive, the proposed fluorometric method for determining α-tocopherol is robust and fairly fast.
Ithas been tested in parallel with a conventional thin layer chromatographic method on foods and feeds.
The only necessarycleanup is the usual saponification.
The unsaponificable fraction can be extracted with ethyl ether or, preferably, withExtrelut columns.
Reagents and their solvents are added to the isooctane solution before each successive reaction and arethen eliminated by partition with water.
The α-tocopherol (α-T) derivative always remains in isooctane.
The first step isnitrosation and elimination of tocopherols and tocotrienols other than α-isomers.
α-T is then oxidized to α-tocored (α-TR)with a mixture of sulfuric acid, ferric chloride, and iodine bromide.
Α-TR is then condensed to a new reagent: 4,5-dimethylo-phenylenediamine.
The phenazine formed is strongly fluorescent.
Iodine and bromine add to the double bonds of α-tocotrienol present and quench the fluorescence of its phenazine.
Related Results
Analysis of the current vitamin A terminology and dietary regulations from vitamin A1 to vitamin A5
Analysis of the current vitamin A terminology and dietary regulations from vitamin A1 to vitamin A5
Abstract: Dietary recommendations on vitamin intake for human food fortification concerning vitamin A in various countries, larger economic zones and international organizations ar...
Effectiveness of vitamin D2 compared with vitamin D3 replacement therapy in a primary healthcare setting: a retrospective cohort study
Effectiveness of vitamin D2 compared with vitamin D3 replacement therapy in a primary healthcare setting: a retrospective cohort study
Introduction: Vitamin D deficiency is a worldwide public health concern, which can lead to severe diseases, such as rickets in children and osteomalacia in adults. Most studies hav...
Comparison of Vitamin A and Vitamin D Levels of 0-36 Months Old Children, Who were Admitted to Pediatric Inpatient Clinic with Lower Respiratory Tract Infection and Healthy Children within the Same Age Group
Comparison of Vitamin A and Vitamin D Levels of 0-36 Months Old Children, Who were Admitted to Pediatric Inpatient Clinic with Lower Respiratory Tract Infection and Healthy Children within the Same Age Group
Background
Lower respiratory system infections are highly common in young children and may require inpatient treatment. Studies have shown that patients treated and followed up fo...
Vitamin D in Critically Ill Patients - From Molecular Damage Interactions to Clinical Outcomes Benefits. When, Why, How?
Vitamin D in Critically Ill Patients - From Molecular Damage Interactions to Clinical Outcomes Benefits. When, Why, How?
Abstract
Vitamin D - „the sunshine vitamin” is essential for the good functioning of the human body. The most important forms of the vitamin D are the vitamin D2 an...
Vitamin D and the Immune System. When? Why? How?
Vitamin D and the Immune System. When? Why? How?
Vitamin D, called “the sunshine vitamin” is essential for the good functioning of the human body. Vitamin D generates its principal effects via the vitamin D receptor (VDR), a spec...
De Novo Anemia and Relationship with Vitamin C Deficiency and Zinc Deficiency in a Southern Delaware Population, a Retrospective Analysis
De Novo Anemia and Relationship with Vitamin C Deficiency and Zinc Deficiency in a Southern Delaware Population, a Retrospective Analysis
Abstract
Background:
Vitamin C is an essential dietary nutrient. It is a water soluble vitamin that exists in the body primarily in the reduced form A...
Importance of vitamin D in critically ill children with subgroup analyses of sepsis and respiratory tract infections: a systematic review and meta-analysis
Importance of vitamin D in critically ill children with subgroup analyses of sepsis and respiratory tract infections: a systematic review and meta-analysis
SummaryBackgroundCritical care and sepsis remain high priority concerns in children. Observational studies report high prevalence of vitamin D deficiency and present mixed results ...
Frequency of Hypovitaminosis D in Obese Adolescent with Polycystic Ovary Syndrome
Frequency of Hypovitaminosis D in Obese Adolescent with Polycystic Ovary Syndrome
Background: Polycystic ovary syndrome (PCOS) is one of the most common female endocrine disorders affecting the reproductive age group and is thought to be one of the leading cause...