Significance of Tumor Mutation Burden Related Immune Gene in the Progression and Prognosis of Clear Cell Renal Cell Carcinoma

Abstract Background: Clear cell renal cell carcinoma (ccRCC) is a common renal malignant disease with a poor prognosis. There were limited studies focus on the relationship between Tumor mutation burden (TMB) and ccRCC.Methods: Based on TCGA-ccRCC cohort, we summarized the status of gene mutations in ccRCC. Then, we analyzed the relationship between TMB and clinical characteristic. Meanwhile, we identified some TMB-related immune genes through the intersection of TMB-Related differentially expressed genes (DEGs) and immune related genes. Finally, we selected the highest correction and novel genes for the future analysis.Results: The most common mutation of Variant Classification, Variant Type, SNV Class were missense mutations, SNP, C>T, respectively. Higher TMB related to shorter overall survival (OS), lower age and grade. Finally, we identified PAEP gene, a novel TMB-related immune gene in ccRCC, which was significantly overexpression in ccRCC tissues and cells with progression and poor survival in ccRCC patients. Furthermore, PAEP promoted the invasion, migration, and proliferation of ccRCC cells. Mechanistically, PAEP suppressed the PI3K/Akt/NF-κB signaling pathway.Conclusion: Our study suggests that PAEP might represents a potential target of antibody immunotherapy for ccRCC patients.