Effect of Shanxian Granule on Immunity in Lewis Lung Cancer Mice

Abstract Objective: To observe the effect of Shanxian Granule on the proliferation of Lewis lung cancer cells and anti-tumor immunity of Lewis lung cancer bearing mice, and explore the molecular mechanism of Shanxian Granule in inhibiting tumors and improving the anti-tumor immunity of the body, so as to provide a profound theoretical basis for its clinical application. Methods: Lewis lung cancer cells were implanted into subaxillary skin to establish tumor bearing mice model, the mice were divided into control group, model group, chemotherapy group and Shanxian granule group, calculating the inhibition rate by weighing the tumor of Lewis lung cancer bearing mice, calculating the spleen index by weighing the spleen, immunohistochemical method was used to detected the expression of lymphocyte CD4+ and CD8+ in spleen. Results: 1. The inhibition rate of Shanxian granule group on Lewis lung cancer was 45.99%, significantly higher than that of chemotherapy group (P < 0.05); 2. Spleen index, ratio of CD4+ T cell and CD8+ T cell in model group and chemotherapy group were significantly lower than those in the control group and Shanxian granule group (P < 0.05); while there was no significant difference between the control group and Shanxian granule group (P < 0.05). Conclusion: Shanxian Granule could obviously inhibit the tumor growth in Lewis lung cancer bearing mice, increase spleen index, enhance T lymphocyte activity and restore immune homeostasis of mice, indicating that the anti-tumor effect of Shanxian Granule may be related to the improvement of immunity and the enhancement of immune surveillance function.