Agrobacterium mediated transformation of Marchantia spores v6

This method produces random integration of DNA into the genome of Marchantia. The technique relies on the capacity of Agrobacterium tumefasciens to transfer DNA to plants. This DNA can be specified as it is flanked by sequences of DNA called the Right and Left Border (RB & LB), named as a whole as the T-DNA. The original tumour-inducing (Ti) plasmid from Agrobacterium was adapted to a binary plasmid system allowing easy cloning of the T-DNA part of the system in E. coli before being electroplated into Agrobacterium and then transfection into plants by infection. This protocol is based onIshizaki et al., 2008 initially set up in Jim Haseloff lab Plant Sciences, University of Cambridge, by Nuri Purswani,Lihua Han and Bernardo Pollak. Further optimised by Linda Silvestri and Susana Sauret-Gueto including feedback from other researchers working in Marchantia, like Bo Xu (Sam Brockington group, Plant Sciences, University of Cambridge) and Victor Jones (Liam Dolan group at University of Oxford).