Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
The Effects of Stromal Vascular Fraction（SVF）Cells and Adipose-Derived Stem Cells (ADSCs) in Full-Thickness Skin Grafts
View through CrossRef
Abstract
Background: To investigate the role and difference of autologous stromal vascular fraction（SVF）cells and allogeneic adipose-derived stem cells(ADSCs) in full-thickness skin graft.Methods: SVF cells and ADSCs were prepared from the inguinal fat pad of the rats, and full-thickness skin on the back of the rats were transplanted in situ. then, SVF, ADSCs and PBS were injected under the graft. Gross survival, H-E staining, Masson staining, CD31 and VEGF immunofluorescence were observed on 3d, 7d and 14d respectively.Results: the survival rate of SVF group was the best, followed by ADSCs group (p<0.05). the H-E and Masson staining structures of the SVF group were better than those of the other two groups under the microscope. CD31 and VEGF expression in SVF group at 7 days and 14 days after surgery was more obvious than that in ADSCs group and PBS group.Conclusion: Subcutaneous injection of autologous SVF or allogeneic ADSCs cells can improve the survival rate of full-thickness skin grafts. In addition, the effect of autologous SVF cells is better than that of allogeneic ADSCs.
Research Square Platform LLC
Title: The Effects of Stromal Vascular Fraction（SVF）Cells and Adipose-Derived Stem Cells (ADSCs) in Full-Thickness Skin Grafts
Description:
Abstract
Background: To investigate the role and difference of autologous stromal vascular fraction（SVF）cells and allogeneic adipose-derived stem cells(ADSCs) in full-thickness skin graft.
Methods: SVF cells and ADSCs were prepared from the inguinal fat pad of the rats, and full-thickness skin on the back of the rats were transplanted in situ.
then, SVF, ADSCs and PBS were injected under the graft.
Gross survival, H-E staining, Masson staining, CD31 and VEGF immunofluorescence were observed on 3d, 7d and 14d respectively.
Results: the survival rate of SVF group was the best, followed by ADSCs group (p<0.
05).
the H-E and Masson staining structures of the SVF group were better than those of the other two groups under the microscope.
CD31 and VEGF expression in SVF group at 7 days and 14 days after surgery was more obvious than that in ADSCs group and PBS group.
Conclusion: Subcutaneous injection of autologous SVF or allogeneic ADSCs cells can improve the survival rate of full-thickness skin grafts.
In addition, the effect of autologous SVF cells is better than that of allogeneic ADSCs.
Related Results
Stem cells
Stem cells
What is a stem cell? The term is a combination of ‘cell’ and ‘stem’. A cell is a major category of living thing, while a stem is a site of growth and support for something else. In...
Differences in the Inflammatory Response of White Adipose Tissue and Adipose-Derived Stem Cells
Differences in the Inflammatory Response of White Adipose Tissue and Adipose-Derived Stem Cells
The application of liposuctioned white adipose tissue (L-WAT) and adipose-derived stem cells (ADSCs) as a novel immunomodulatory treatment option is the currently subject of variou...
Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis
Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis
AbstractMultiple sclerosis (MS) is a common neurodegenerative disease and remains an unmet clinical challenge. In MS, an autoimmune response leads to immune cell infiltration, infl...
e0142 The effects of stromal vascular fraction from adipose tissue on rat acute myocardial infarction
e0142 The effects of stromal vascular fraction from adipose tissue on rat acute myocardial infarction
Objective
To observe the effects of transplantation of cultured stromal vascular fraction (SVF) on infarcted myocardial tissue, cardiac function and the underling...
IMPROVEMENT OF CARDIAC FUNCTION BY TRANSPLANTED MESENCHYMAL STEM CELLS WITH DOWN-REGULATION OF PHD2 VIA PARACRINE FACTORS IN MYOCARDIAL INFARCTED MICE
IMPROVEMENT OF CARDIAC FUNCTION BY TRANSPLANTED MESENCHYMAL STEM CELLS WITH DOWN-REGULATION OF PHD2 VIA PARACRINE FACTORS IN MYOCARDIAL INFARCTED MICE
Objectives
Transplantation of stem cell has emerged as a promising therapeutic intervention for cardiac repair. However, the survival rate of post-transplanted st...
Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties
Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties
Beneficial effects have been observed following the transplant of lipoaspirates containing adipose-derived stem cells into chronic wounds caused by oncologic radiotherapy. It is no...
Histological Study on the Protective Role of Intranasally Applied Adipose-Derived Mesenchymal Stem Cells on Demyelinated Corpus Callosum in A Mouse Model of Multiple Sclerosis
Histological Study on the Protective Role of Intranasally Applied Adipose-Derived Mesenchymal Stem Cells on Demyelinated Corpus Callosum in A Mouse Model of Multiple Sclerosis
Abstract
Introduction
multiple sclerosis (MS) is the most common demyelinating disease that leads to non-traumatic disability. I...
Intra-articular adipose-derived cell therapies for knee osteoarthritis: a systematic review of randomized controlled trials
Intra-articular adipose-derived cell therapies for knee osteoarthritis: a systematic review of randomized controlled trials
Introduction
Knee osteoarthritis is a leading cause of chronic pain, functional limitation, and disability worldwide, imposing a substantial socioeconomic burde...