Supplementary figures from miRNA-487a Promotes Proliferation and Metastasis in Hepatocellular Carcinoma

<p>Figure S1. Diagrammatic sketch of patients enrolled and groups designed. The detailed description was supplied in Method and Materials. Figure S2. In situ hybridization showed the miR-487a expression in HCC tissues. Figure S3. The infective efficiency of lentivirus in HCC cells. Figure S4. miR-487a promotes proliferation of HCC cells. Figure S5. miR-487a promotes proliferation and metastasis of MHCC97H and PLC/PRF/5. Figure S6. Protein and mRNA expression of SPRED2 and PIK3R1 in HCC tissues. Figure S7. miR-487a promotes proliferation via PIK3R1, but not SPRED2. Figure S8. miR-487a promotes proliferative or metastatic activity of HCC cells via PIK3R1 or SPRED2. Figure S9. miR-487a may promote proliferation or metastasis by regulating PIK3R1 mediated p-AKT or SPRED2 mediated p-ERK. Figure S10. Silencing of miR-487a suppresses HCC tumor growth and metastasis in vivo. Figure S11. The SPRED2, p-ERK, PIK3R1, p-AKT expression in serials sections of tumors collected from mice of HCCLM3Anti-miR-487a group and HCCLM3NC group. Figure S12. Schematic representation of the role and molecular mechanism of miR-487a in HCC. Figure S13. HCCLM3 cell labeled with luciferase is detectable by IVIS system. Figure S14. Morpholino-anti-miR-487a effectively suppresses the metastasis of HCC cells in HCC lung metastasis mouse model.</p>