ENZYME-ASSISTED EXTRACTION OF TRITERPENOID AND PHENOLIC COMPOUNDS FROM Rubus alceaefolius Poir LEAVES

The leaves of Rubus alceaefolius Poir primarily consist of phenolic and triterpenoid compounds, which possess a variety of biological activities beneficial to human health. This research aimed to explore the impact of enzyme-assisted extraction conditions on the recovery of triterpenoid and phenolic compounds from Rubus alceaefolius Poir leaves. Factors considered in the enzymatic treatment process with Viscozyme L cellulase included the solid-to-water ratio (1:15-1:45 w/v), enzyme concentration (0.5-3.5%), temperature (40-60 °C), and extraction duration (30-180 min). The results suggested that the optimal conditions for the enzymatic extraction of triterpenoid and phenolic compounds were a 2.5% enzyme concentration, a 1:35 (w/v) solid-to-solvent ratio, an incubation temperature of 50 °C, and an extraction time of 120 minutes. Under these conditions, the highest amounts of total triterpenoids and phenolics obtained were 231.45 mg UA/g and 9.13 mg GAE/g, respectively. GC-MS analysis identified 17 constituents that potentially contribute to antioxidant potency. Furthermore, the findings showed that the enzyme-assisted extraction (EAE) technique was more efficient in extracting total phenolics and triterpenoids compared to conventional maceration extraction.