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IN VITRO MORPHOGENETIC REACTION OF MELISSA OFFICINALIS L.
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Lemon balm (Melissa officinalisL.) is a medicinal plant with a long history in
traditional medicine. Classical propagation of this species is inefficient for
establishing a good quality clonal plants. The aim of this work was to elaborate an in
vitro propagation protocol for M. officinalis using apexes and uninodal fragments as
explants. The highest multiplication rate (4.7 shoots/explant) was obtained on a MS
medium supplemented with 3 mg/L BAP. A half strength MS medium supplemented
with 1 mg/L NAA was the most effective for in vitro rooting of lemon
balmmicroshoots. Micropropagated plants transferred ex vitro showed normal
morphology and 95% survival rate during acclimatization. The results obtained
throughout the in vitro regeneration phases confirm that in vitro tissue culture is an
efficient method for multiplication of M. officinalis.
Academia de Stiinte Agricole si Silvice 'Gheorghe Ionescu-Sisesti'
Title: IN VITRO MORPHOGENETIC REACTION OF MELISSA OFFICINALIS L.
Description:
Lemon balm (Melissa officinalisL.
) is a medicinal plant with a long history in
traditional medicine.
Classical propagation of this species is inefficient for
establishing a good quality clonal plants.
The aim of this work was to elaborate an in
vitro propagation protocol for M.
officinalis using apexes and uninodal fragments as
explants.
The highest multiplication rate (4.
7 shoots/explant) was obtained on a MS
medium supplemented with 3 mg/L BAP.
A half strength MS medium supplemented
with 1 mg/L NAA was the most effective for in vitro rooting of lemon
balmmicroshoots.
Micropropagated plants transferred ex vitro showed normal
morphology and 95% survival rate during acclimatization.
The results obtained
throughout the in vitro regeneration phases confirm that in vitro tissue culture is an
efficient method for multiplication of M.
officinalis.
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