In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells

OBJECTIVE: To evaluate the action of risedronate and cobalamin, and effects when associated, when administered osteoblastic cells. METHODS: The MC3T3 cells were cultivate in the media α-MEM and α-MEM supplemented with mineralizing factors, ascorbic acid and disodium α-glyicerophosphate, and treated with risedronate, cobalamin, and risedronate associated with cobalamin in a concentration of 10-3 M. The cell proliferation and formation of calcium and phosphate nodules were evaluated at 24 hours, 48 hours, 72 hours, 5 days and 7 days via a Neubauer Chamer count, Alizarin and the von Kossa reaction. RESULTS: The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. CONCLUSION: The conclusion was reached that using risedronate, cobalamin and both drugs in combination on osteoblastic cell cultures does not cause alterations to their growth or in the formation of calcium and phosphate nodules.