Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Detection and Elimination of Four Viruses in Carnation (Dianthus caryophyllus L.)
View through CrossRef
Carnation (Dianthus caryophyllus L.) is one of the most popular cut flowers throughout the world. However, it is seriously damaged by virus infection. Four viruses were detected and eliminated from three popular carnation cultivars (‘Master’, ‘Pink’ and ‘Liberty’). The coat protein gene sequence-based primers were designed and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was optimized for the detection of four viruses: Carnation mottle virus (CarMV), Carnation ring spot virus (CRSV), Carnation latent virus (CLV) and Carnation necrotic fleck virus (CNFV). The detection sensitivity level of this method was up to pg 10-12. The PCR product homologous sequences were compared with most isolate nucleotides in GenBank, and no significant differences were found. The nucleotide sequence homology of CarMV, CRSV, CLV and CNFV was more than 88%. Chemotherapy, thermotherapy and microapical culture were applied to eliminate viruses, and virus-free plants from the three cultivars were obtained. Chemotherapy was found to be the most efficient way for virus elimination due to its short duration, high efficiency and practicability.
College of Agriculture and Food Science, University of the Philippines Los Banos
Title: Detection and Elimination of Four Viruses in Carnation (Dianthus caryophyllus L.)
Description:
Carnation (Dianthus caryophyllus L.
) is one of the most popular cut flowers throughout the world.
However, it is seriously damaged by virus infection.
Four viruses were detected and eliminated from three popular carnation cultivars (‘Master’, ‘Pink’ and ‘Liberty’).
The coat protein gene sequence-based primers were designed and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was optimized for the detection of four viruses: Carnation mottle virus (CarMV), Carnation ring spot virus (CRSV), Carnation latent virus (CLV) and Carnation necrotic fleck virus (CNFV).
The detection sensitivity level of this method was up to pg 10-12.
The PCR product homologous sequences were compared with most isolate nucleotides in GenBank, and no significant differences were found.
The nucleotide sequence homology of CarMV, CRSV, CLV and CNFV was more than 88%.
Chemotherapy, thermotherapy and microapical culture were applied to eliminate viruses, and virus-free plants from the three cultivars were obtained.
Chemotherapy was found to be the most efficient way for virus elimination due to its short duration, high efficiency and practicability.
Related Results
Distinguish Dianthus species or varieties based on chloroplast genomes
Distinguish Dianthus species or varieties based on chloroplast genomes
Abstract
Most plants belonging to the widely distributed genus Dianthus are used for gardening. Interspecific hybridization of different Dianthus species leads to bl...
Effects of Carnation Essential Oil Extracted from Carnation Calli on Extending Shelf Life of Yoghurt
Effects of Carnation Essential Oil Extracted from Carnation Calli on Extending Shelf Life of Yoghurt
Carnation’s essential oil with high content of eugenol (monoterpenes) was extracted from carnation plant calli. Eugenol had antimicrobial effect and could be used to prolong the sh...
Fungitoxic phenols from carnation (Dianthus caryophyllus) effective against Fusarium oxysporum f. sp. dianthi
Fungitoxic phenols from carnation (Dianthus caryophyllus) effective against Fusarium oxysporum f. sp. dianthi
AbstractThe phenol compositions of two cultivars of carnation (Dianthus caryophyllus) namely “Gloriana” and “Roland”, which are partially and highly resistant, respectively, to Fus...
Characterisation and zoonotic risk of tick viruses in public datasets
Characterisation and zoonotic risk of tick viruses in public datasets
AbstractTick-borne viruses remain a substantial zoonotic risk worldwide, so knowledge of the diversity of tick viruses has potential health consequences. Despite their importance, ...
CITRA TOKOH UTAMA PEREMPUAN DAN NILAI PENDIDIKAN KARAKTER DALAM NOVEL CARNATION KARYA SANAZ NADYA
CITRA TOKOH UTAMA PEREMPUAN DAN NILAI PENDIDIKAN KARAKTER DALAM NOVEL CARNATION KARYA SANAZ NADYA
Karya sastra merupakan cerminan dari kehidupan manusia, salah satunya perempuan yang beragam watak sifat di dalam kesusastraan itu sendiri. Dalam sebuah karya sastra juga memiliki ...
Common but Nonpersistent Acquisitions of Plant Viruses by Plant-Associated Fungi
Common but Nonpersistent Acquisitions of Plant Viruses by Plant-Associated Fungi
Investigating a virus’s host range and cross-infection is important for better understanding the epidemiology and emergence of viruses. Previously, our research group discovered a ...
Comparative Study of Qualitative Phytochemical Constituents of Calotropis procera Leaves, Latex, and Flowers, Zanthoxylum armatum Dried Seeds, and Eugenia caryophyllus Dried Flower Buds
Comparative Study of Qualitative Phytochemical Constituents of Calotropis procera Leaves, Latex, and Flowers, Zanthoxylum armatum Dried Seeds, and Eugenia caryophyllus Dried Flower Buds
Objective: This study aimed to conduct a comparative qualitative analysis of phytochemical constituents in various parts of Calotropis procera (leaves, latex, and flowers), Zanthox...