P-505 Autologous platelet-rich plasma (PRP) intrauterine application during the assisted reproduction technique

Abstract Study question Does the use of PRP infused into the endometrium of women undergoing IVF promote endometrial receptivity and embryo implantation? Summary answer The promotion of cell proliferation and other growth factors aids in endometrial receptivity, facilitating embryo implantation and contributing to a positive pregnancy outcome. What is known already The endometrium is a dynamic tissue capable of growth and differentiation in response to hormonal regulation. Through cell proliferation and growth factors, the tissue attains a functional state that allows for receptivity and embryo implantation. Study design, size, duration A prospective study was conducted with 890 participants divided into two groups: 519 control patients (C) and 371 volunteers for PRP application (PRP). The study was approved by the Ethics Committee (58399022.4.0000.5505) and conducted over four years. After signing the informed consent and meeting the inclusion and exclusion criteria, participants had their blood drawn, which was centrifuged in two steps to produce autologous PRP. Participants/materials, setting, methods All participants underwent controlled ovarian stimulation, and only PRP group received the prepared PRP via uterine infusion. The retrieved oocytes were injected using the ICSI technique, to both groups. The blastocysts were transferred to the uterus. Follicle, oocyte, fertilization, embryonic development, and pregnancy rates were compared between the groups. For statistical analysis, with age as a control variable, the logistic and linear regression was applied for continuous variables, with α = 5%. Main results and the role of chance According to the exclusion criteria, 487 women remained in the C group, and 361 in the PRP group; however, only 10% of them progressed to embryo transfer. Although the number of blastocysts (4.65 vs. 3.27, p = 0.024), Grade A D3 embryos (2.34 vs. 1.97, p = 0.019), blastocyst rate (0.13 vs. 0.05, p < 0.001), and the number of transferred embryos (2.54 vs. 2.34, p < 0.001) were higher in the group of women who received PRP, the number of live births was lower (0.28 vs. 2.00, p = 0.024). Limitations, reasons for caution The limitations include the small percentage of participants progressing to embryo transfer. Additionally, confounding factors beyond age might affect results, and long-term effects were not evaluated. Further research with larger sample sizes with placebo group and standardized methods is needed to confirm PRP’s efficacy and safety. Wider implications of the findings Autologous PRP suggests potential benefits in improving embryo quality and endometrial receptivity, which could enhance IVF protocols. However, the lower live birth rate indicates the need for caution. The study highlights the importance of standardizing PRP preparation, understanding long-term safety, and conducting further research. Trial registration number No