CORRELATION OF HAEMATOLOGICAL PROFILE AND SICKLE CELL DISEASE: A STUDY IN RAIPUR AND DURG DISTRICTS OF CHHATTISGARH

Sickle cell disease is an inherited structural disorder of hemoglobin and a major public health issue in the state of Chhattisgarh, India. Government of Chhattisgarh passed ‘Sickle cell bill’ in the Assembly looking to its previous impact on society. The present study was executed on sickle cell disease patients and controls. A cross-sectional study was done for screening of sickle cell hemoglobin with individual informed written consent, 90 subjects of Raipur & Durg districts, belonging to different caste category, ranging in age between 1.5year to 45 year were screened using 2% sodium meta bisulphate solution. Genotype of individuals with sickle cell hemoglobin as done by Allele specific polymerase chain reaction (ASPCR) analysis and normal individuals were treated as control. ERMA total blood cell counter machine (PCE-210) was used for the analysis of various hematological parameters. The study focused on two aspects. Firstly, mutant beta chain of HbSS was distinguished form normal beta chain of HbAA by using technique of PCR followed by gel electrophoresis. Secondly HbSS, HbAS & HbAA individuals correlated with various hematological indices. We found significant statistical difference for hematological indices – DLC, Leucocytes, bleeding time & clotting time between Sickle cell patients (HbSS & HbAS) & controls.