e0401 Changes of intracellular calcium concentration in cardiac-like myocytes

Objective To study the effects of verapamil, endothelin on [Ca2+]i in cardiac-like myocytes derived of bone marrow mesenchymal stem cells. Methods (1) Bone marrow mesenchymal stem cells and cardiac myocytes were cultured by primary method; (2) Bone marrow mesenchymal stem cells differentiated into cardiac-like myocytes by 5-azacytidine induction agent; (3) We divide our test into three groups, including the first generation bone marrow mesenchymal stem cells, cardiac-like myocytes and cardiac myocytes; (4) The alteration of [Ca2+]i affected by verapamil, endothelins was observed with [Ca2+]i fluorescence imagine system in bone marrow mesenchymal stem cells, cardiac-like myocytes and cardiac myocytes. Results (1) Cardiac myocytes were set up through trypsogen digestion method; (2) The alteration of [Ca2+]i affected by verapamil was observed with [Ca2+]i fluorescence imagine system. There was a same trend of variability between cardiac-like myocytes and cardiac myocytes, but no changes in bone marrow mesenchymal stem cells; (3) The alteration of [Ca2+]i affected by endothelin was observed with [Ca2+]i fluorescence imagine system. There was a same trend of variability that fluorescence intensity gradually strengthened with intervention time extended. Conclusions After affected by verapamil and endothelins, there was a same trend of [Ca2+]i changes between cardiac-like myocytes from bone marrow mesenchymal stem cells and cardiac myocytes. It shows that some common electrophysiological characteristics exist in cardiac-like myocytes and cardiaomyocytes.