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PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle
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Meat authentication is currently a key topic in relation to the quality and safety of food of animal origin at all levels of production and the global distribution chain. New polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) based on digestion of PCR products with two restriction enzymes, MboII and AciI, have been developed for the specific identification of raw and heat-processed meat from red deer (
Cervus elaphus
), sika deer (
Cervus nippon
), roe deer (
Capreolus capreolus
), fallow deer (
Dama dama
), mouflon (
Ovis musimon
), wild boar (
Sus scrofa
), hare (
Lepus europaeus
) and cattle (
Bos taurus
). The PCR primers were targeted in a well-conserved region of the cytochrome
b
(
CYTB
) gene to amplify a 378 bp region of all the analysed species. This simple, rapid and cost-effective method is suitable for identification of the meat of game species and their possible substitution by beef.
University of Veterinary Sciences Brno
Title: PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle
Description:
Meat authentication is currently a key topic in relation to the quality and safety of food of animal origin at all levels of production and the global distribution chain.
New polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) based on digestion of PCR products with two restriction enzymes, MboII and AciI, have been developed for the specific identification of raw and heat-processed meat from red deer (
Cervus elaphus
), sika deer (
Cervus nippon
), roe deer (
Capreolus capreolus
), fallow deer (
Dama dama
), mouflon (
Ovis musimon
), wild boar (
Sus scrofa
), hare (
Lepus europaeus
) and cattle (
Bos taurus
).
The PCR primers were targeted in a well-conserved region of the cytochrome
b
(
CYTB
) gene to amplify a 378 bp region of all the analysed species.
This simple, rapid and cost-effective method is suitable for identification of the meat of game species and their possible substitution by beef.
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