Evaluation of total polyphenol content, total flavonoid content, and antioxidant activity of Centella asiatica

Abstract Nowadays, the tendency to focus on plant research and their biological activities has increased worldwide. Centella asiatica is a crucial herb widely used in East Asia and is gaining popularity in the West. In Vietnam, besides being used as daily food, it is also an herb that is recommended to treat various skin disorders include leprosy, lupus, varicose ulcers, eczema, female genital diseases and also help reduce anxiety. The aerial parts are also proven to be scientific cash in the biological activities contained in it with broad therapeutic applications in the antibacterial, anti-inflammatory, and so on. This research aimed to assess the quality of phytochemicals, TPC and TFC, and antioxidant activities of C. asiatica (L). Moreover, antioxidant activity was conducted through radical scavenging ABTS and DPPH. Under purified water and 96% ethanol, the phytochemical was collected separately. The findings indicate that C. asiatica (L) produced an extensive range of pharmacologically active substances, including alkaloid, tannins, flavonoids, terpenoids, and reducing sugar. TPC and TFC of ethanol extract in C. asiatica leave achieved 2.14±0.29 mgGAE/g and 23.03±2.89 mgQE/g, respectively. The total polyphenol content of ethanolic and water extraction was different, achieved 2.14 ± 0.29 (mg GAE/g) and 2.82 ± 1.68 (mg GAE/g), respectively. Moreover, the TFC of water extract (30.09 ± 2.67 mg QE/g) was significantly higher than that of ethanolic extract (23.03 ± 2.89 mg QE/g). The antioxidant activity of the C. Asiatica was correlated with total phenolic and flavonoid content with values IC50 achieving 2324.26 µg/ml in aqueous extract, and 1744.77 µg/ml in Ethanolic extract. The results showed that leaves of C. asiatica (L) were a valuable source to exploit metabolic compounds primary and secondary are valid.