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Construction and properties of a triprotein containing the high‐affinity nickel transporter of Alcaligenes eutrophus
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The high‐affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1. A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for β‐galactoxidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A. eutrophus gene hoxN. An E. coli strain harboring this construct (plasmid pCH307) efficiently produced the corresponding triprotein upon induction. A broad‐host‐range derivative of pCH307 was shown to complement an A. eutrophus HoxN− mutant.
Title: Construction and properties of a triprotein containing the high‐affinity nickel transporter of Alcaligenes eutrophus
Description:
The high‐affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1.
A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for β‐galactoxidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A.
eutrophus gene hoxN.
An E.
coli strain harboring this construct (plasmid pCH307) efficiently produced the corresponding triprotein upon induction.
A broad‐host‐range derivative of pCH307 was shown to complement an A.
eutrophus HoxN− mutant.
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