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Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
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Abstract
Objective
This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection.
Results
About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing.
Springer Science and Business Media LLC
Title: Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
Description:
Abstract
Objective
This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection.
Results
About 6.
2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.
7% (7/80) of samples were changed to negative in heat inactivation at 100 °C.
The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C.
The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration.
The efficacy of heat-inactivation varies greatly depending on temperature and duration.
Local validation of heat-inactivation and its effects is therefore essential for molecular testing.
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Effect of Heat inactivation and bulk lysis on Real-Time Reverse Transcription PCR Detection of the SARS-COV-2: An Experimental Study
Abstract
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