Association Between BsmI Vitamin D Receptor Gene Polymorphism and Clinicopathological Features of Colorectal Cancer Patients in 2019-2020 at Prof. Dr. I.G.N.G Ngoerah Denpasar Bali

Aging is related to the occurrence of cancer. Colorectal cancer is one of the most common types of cancer found in Indonesia. The BsmI vitamin D receptor polymorphism has been extensively studied before and has been associated with colorectal cancer. This study aims to determine the relationship between the BsmI vitamin D receptor polymorphism and the clinicopathological features of colorectal cancer patients in 2019-2020 at Prof. dr. I.G.N.G Ngoerah Denpasar Bali. This research is a cross-sectional analytical research. The study was conducted at the Biomedical Laboratory Unit of the Faculty of Medicine, Udayana University from June to December 2022. A total of 35 samples were obtained from stored biological materials of colorectal cancer patients who were treated at the Digestive Surgery Polyclinic at Prof. RSUP. dr. I.G.N.G Ngoerah in 2019-2020 in the Udayana University Biochemistry laboratory. To detect the status of the BsmI polymorphism, DNA segments were amplified by the polymerase chain reaction (PCR) method and followed by sequencing. The results showed from 35 samples, 8 samples (22.9%) had the GG allele and 27 samples (77.1%) had the GA allele. A statistically significant association was found between BsmI vitamin D receptor polymorphisms and colorectal cancer grading (p=0.016), but no significant association was found between BsmI vitamin D receptor polymorphisms and the histopathological type of colorectal cancer (p=0.553). The conclusion of this study is that there is a significant relationship between BsmI vitamin D receptor polymorphism and grading, but not with the histopathological type of colorectal cancer, age and gender of colorectal cancer patients in 2019-2020 at Prof. dr. I.G.N.G Ngoerah Denpasar Bali. Keywords: Colorectal cancer, Vitamin D receptor, BsmI polymorphism, clinicopathology.