Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Evaluation of P1' substrate specificity of staphylococcal SplB protease.
View through CrossRef
Staphylococcus aureus is a dangerous human pathogen characterized by growing antibiotic resistance. Virulence of S. aureus relies on a variety of secreted and cell surface associated virulence factors among which certain proteolytic enzymes play an important role. Amid staphylococcal extracellular proteases, those encoded by the spl operon remain poorly characterized, both in terms of enzymology and their physiological role. Initial data demonstrated that Spl proteases exhibit restricted substrate specificity. This study describes development of convenient protein FRET substrates for SplB protease and characterization of the substrate preference of the protease at the P1' position. Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided.
Frontiers Media SA
Title: Evaluation of P1' substrate specificity of staphylococcal SplB protease.
Description:
Staphylococcus aureus is a dangerous human pathogen characterized by growing antibiotic resistance.
Virulence of S.
aureus relies on a variety of secreted and cell surface associated virulence factors among which certain proteolytic enzymes play an important role.
Amid staphylococcal extracellular proteases, those encoded by the spl operon remain poorly characterized, both in terms of enzymology and their physiological role.
Initial data demonstrated that Spl proteases exhibit restricted substrate specificity.
This study describes development of convenient protein FRET substrates for SplB protease and characterization of the substrate preference of the protease at the P1' position.
Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided.
Related Results
SERUM ANTIBODIES IN STAPHYLOCOCCAL DISEASE
SERUM ANTIBODIES IN STAPHYLOCOCCAL DISEASE
Serum antibody titers to the staphylococcal Muller factor and alpha-hemolysin were determined in population groups of various ages and in maternal-cord serum pairs. There was evide...
Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent
Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent
Abstract
BackgroundA wide variety of Bacterial species produces protease enzyme and the application of same enzyme have been manipulated precisely and used in various biote...
The treatment of resistant staphylococcal infections
The treatment of resistant staphylococcal infections
Staphylococcus aureus of the many staphylococcal species is the most common cause of both skin and soft tissue infection and severe staphylococcal infections including Staphylococc...
Isolasi dan Karakterisasi Bakteri Termofililk Penghasil Enzim Protease Asal Sumber Air Panas Way Belerang, Lampung
Isolasi dan Karakterisasi Bakteri Termofililk Penghasil Enzim Protease Asal Sumber Air Panas Way Belerang, Lampung
Abstrak
Protease merupakan enzim yang menghidrolisis ikatan peptida pada protein. Enzim ini biasa dimanfaatkan dalam industri pangan, pertanian, medis hingga membantu dalam proses...
Abstract 1577: Inside-out regulation of ectodomain protease accessibility in the release of cytokines
Abstract 1577: Inside-out regulation of ectodomain protease accessibility in the release of cytokines
Abstract
Growth factors of the EGF family and numerous other growth factors and cytokines are produced as transmembrane precursor proteins and released by proteolysi...
Nitric Oxide Inhibits Dystrophin Proteolysis by Coxsackieviral Protease 2A Through
S
-Nitrosylation
Nitric Oxide Inhibits Dystrophin Proteolysis by Coxsackieviral Protease 2A Through
S
-Nitrosylation
Background
—Infection with enteroviruses like coxsackievirus B3 (CVB3) as well as genetic dystrophin deficiency can cause dilated cardiomyopathy. We recently identified...
Purification and Characterization of Vibrio vulnificus Protease
Purification and Characterization of Vibrio vulnificus Protease
AbstractA protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone‐yeast extract medium, and...
How to find simple and accurate rules for viral protease cleavage specificities
How to find simple and accurate rules for viral protease cleavage specificities
BACKGROUND:Proteases of human pathogens are becoming increasingly important drug targets, hence it is necessary to understand their substrate specificity and to interpret this know...