Comparative Estimation of Plumbagin in Aerial and Root Part of Plumbago zeylanica Using UV-Visible Spectrophotometric

Plumbago zeylanicais one such important medicinal plant which is being used the world over in the traditional system of medicines. The plant is being used extensively in commercial preparations of medicines. The plumbagin present in different parts of Plumbago zeylanica as active constituent and it imparts wide range of biological activities. In the present study we planned to check the concentration of plumbagin present in aerial and root of Plumbago zeylanica. The petroleum ether, ethanol and aqueous extracts were prepared from the aerial and root of Plumbago zeylanica by successive extraction method. The phytochemical investigation of Plumbago zeylanica exhibited maximum phytoconstituents along flavonoids and polyphenol present in ethanol and aqueous extracts. While the petroleum ether extract of aerial and root contains steroid. The Plumbagin content in ethanol extract of aerial and root of Plumbago zeylanica was estimated from the calibration graph plotted from pure plumbagin. The findings of study indicate that concentration of plumbagin in aerial part of Plumbago zeylanica is maximum compared to root.