RELEASE OF APPLE EXTRACT AND PHENOLIC COMPOUNDS FROM FIVE SEMI-SOLID FORMULATIONS

The clinical effectiveness of the phenolic compounds from apple extract will depend not only on their activity potency but also on the achieve of their action targets, and to achieve local biological effect from designed topical formulations, active substances have to be released from it. The aim of our study was to perform release test in vitro of emulsion, emulgel, gel, ointment, and oleogel containing an apple extract and individual phenolic compounds solutions. The release of phenolic compounds from semi-solid forms was assessed by Franz-type diffusion cells. The phenolic compounds of apple extract, and pure individual phenolic compounds released from five different semi-solid formulations results arrange in the following order: oleogel< ointment< gel< emulgel< emulsion. The in vitro release test showed that after 6 hours analysis the highest amount (70.6%) of phenolic compounds was released from emulsion, the lowest contents 31.6% and 29.2% were released from ointment and oleogel, accordingly. We found, that chlorogenic acid was the predominant phenolic compound released from the five different semi-solid forms after 1 ̶ 6 hours. The physicochemical properties of the investigated semi-solid formulations might have influenced the content of phenolic compounds released.