Preparation of Berberin Proliposomes by Film Deposition on Carrier Surface Method

This study aims to formulate berberin (BBR) proliposomes by film-deposition on carrier surface to increase BBR’s solubility and permeability through biological membranes. Proliposomes were hydrated in water to form BBR liposomes for determining the size and distribution of the vesicles. Differential thermal analysis was used to evaluate the BBR proliposomes. The study results show that berberin proliposomes prepared with hydrogenated soy phosphatidylcholine: cholesterol: berberin with a molar ratio of 9:1:6 using sorbitol as carrier with a weight ratio to lipid of 10:1. The obtained BBR proliposomes in the form of a dry yellowish powder were hydrated in water to form BBR liposomes with an average diameter of about 8.41μm. The results of the differential thermal analysis show that BBR was dispersed in molecular form into proliposomes. Keywords: Berberin, proliposomes, sorbitol, film-deposition on the carrier method. References [1] J. Plessis, C. Ramachandran, N. Weiner, D.G Müller, The influence of lipid composition and lamellarity of liposomes on the physical stability of liposomes upon storage, International Journal of Pharmaceutics, 2 (1996) 273-278. https://doi.org/10.1016/0378-5173(95)04281-4[2] A.V. Yadav, M.S. Murthy, Stability Aspects of Liposomes, Indian Journal of Pharmaceutical Education and Research 24 (2011) 402413 – 43. [3] V. Nekkanti, N. Venkatesan, G.V. Betageri, Proliposomes for Oral Delivery: Progress and Challenges, Current Pharmaceutical Biotechnology, 16(2015) 303-312. 10.2174/1389201016666150118134256[4] M. Khayam, S. Umar, Berberine nanoparticles with enhanced in vitro bioavailability: characterization and antimicrobial activity, Drug Design, Development and Therapy, 12 (2018) 303-312. https://doi.org/10.2147/DDDT.S156123[5] S.J. Jia, G.Y. Ningning, L. Zhang, Y. Zhao, Release-controlled curcumin proliposome produced by ultrasound-assisted supercritical antisolvent method, Journal of Supercritical Fluids, 113 (2016) 150-157. https://doi.org/10.1016/j.supflu.2016.03.026.[6] K.G.B. Sharan, R.V. Prabhakar, Formulation, evaluation, and pharmacokinetics of isradipine proliposomes for oral delivery, Journal of liposome research, 4(2012) 285-294. https://doi.org/10.3109/08982104.2012.697067.[7] Q. Fu, H.L. Fu, L. Huan, Preparation of cefquinome sulfate proliposome and its pharmacokinetics in rabbit, Iranian journal of pharmaceutical research, 4 (2013) 611-21. [8] T.T. H. Yen, T.T. Loan, D.T. Thuan, P.T.M. Hue, Preparation of berberin liposomes by ethanol injection method, Pharmaceutical journal, 59 (2019) 54-58 (in Vietnamese). [9] P. Elahehnaz, R. Marzieh, K. Maryam, Design and development of vitamin C-encapsulated proliposome with improved in-vitro and ex-vivo antioxidant efficacy, Journal of microemulsion 3(2018) 301 – 311. https://doi.org/10.1080/02652048.2018.1477845[10] I. Khan, S. Yousaf, S. Subramanian, Proliposome tablets manufactured using a slurry-driven lipid-enriched powders: Development, characterization and stability evaluation, J Int Pharm 1-2 (2018) 250 – 262. doi: 10.1016/j.ijpharm.2017.12.049[11] N.I. Payne, P. Timmins, V.A. Cheryl, Proliposomes: A Novel Solution to an Old Problem, Journal of Pharmaceutical Sciences 4 (1986) 325-329. https://doi.org/10.1002/jps.2600750402.