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Simultaneous Separation and Determination of Vanillin and O-Vanillin by CE Compared with HPLC
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A method for the simultaneous separation and determination of vanillin ando-vanillin by capillary electrophoresis (CE) was developed. The influence of type, concentration and pH of running buffer, and applied voltage on separation was investigated. Under the condition of 50 mmol/L borax-150 mmol/L disodium hydrogen phosphate (pH 7.5) and applied voltage of 15 kV, the vanillin ando-vanillin were separated in less than 6 min. The method was validated in terms of accuracy, precision and linearity and proved to be robust. This CE method is comparable with high performance liquid chromatography (HPLC). This CE method is applicable to the quantitative analysis of Vanillin ando-vanillin in real samples, and the assay results were satisfactory
Title: Simultaneous Separation and Determination of Vanillin and O-Vanillin by CE Compared with HPLC
Description:
A method for the simultaneous separation and determination of vanillin ando-vanillin by capillary electrophoresis (CE) was developed.
The influence of type, concentration and pH of running buffer, and applied voltage on separation was investigated.
Under the condition of 50 mmol/L borax-150 mmol/L disodium hydrogen phosphate (pH 7.
5) and applied voltage of 15 kV, the vanillin ando-vanillin were separated in less than 6 min.
The method was validated in terms of accuracy, precision and linearity and proved to be robust.
This CE method is comparable with high performance liquid chromatography (HPLC).
This CE method is applicable to the quantitative analysis of Vanillin ando-vanillin in real samples, and the assay results were satisfactory.
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