Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Effect of calcium chelators on the Ca2+-dependent luminescence of aequorin
View through CrossRef
The luminescence of aequorin, a useful tool for studying intracellular Ca2+, was recently found to be inhibited by the free EDTA and EGTA that are present in calcium buffers. In the present study we have examined the effect of the free forms of various chelators in the calibration of [Ca2+] with aequorin. Free EDTA and EGTA in low-ionic-strength solutions strongly inhibited the Ca2+-triggered luminescence of aequorin, causing large errors in the calibration of [Ca2+] (approx. 2 pCa units), whereas in solutions containing 150mM-KCl, errors were relatively small (0.2-0.3 pCa units). Citric acid in low-ionic-strength solutions and [(carbamoylmethyl)imino]diacetic acid in high-ionic-strength solutions showed no inhibition and did not cause detectable error in the calibration of [Ca2+], indicating that they are better chelators than EDTA and EGTA for use with aequorin.
Title: Effect of calcium chelators on the Ca2+-dependent luminescence of aequorin
Description:
The luminescence of aequorin, a useful tool for studying intracellular Ca2+, was recently found to be inhibited by the free EDTA and EGTA that are present in calcium buffers.
In the present study we have examined the effect of the free forms of various chelators in the calibration of [Ca2+] with aequorin.
Free EDTA and EGTA in low-ionic-strength solutions strongly inhibited the Ca2+-triggered luminescence of aequorin, causing large errors in the calibration of [Ca2+] (approx.
2 pCa units), whereas in solutions containing 150mM-KCl, errors were relatively small (0.
2-0.
3 pCa units).
Citric acid in low-ionic-strength solutions and [(carbamoylmethyl)imino]diacetic acid in high-ionic-strength solutions showed no inhibition and did not cause detectable error in the calibration of [Ca2+], indicating that they are better chelators than EDTA and EGTA for use with aequorin.
Related Results
Ca2+ entry through Na(+)‐Ca2+ exchange can trigger Ca2+ release from Ca2+ stores in Na(+)‐loaded guinea‐pig coronary myocytes.
Ca2+ entry through Na(+)‐Ca2+ exchange can trigger Ca2+ release from Ca2+ stores in Na(+)‐loaded guinea‐pig coronary myocytes.
1. The ionized cytosolic calcium concentration ([Ca2+]i) was monitored in voltage‐clamped coronary myocytes at 36 degrees C and 2.5 mM [Ca2+]o using the Ca2+ indicator indo‐1. [Ca2...
Computational analysis of Ca2+ dynamics in isolated cardiac mitochondria predicts two distinct modes of Ca2+ uptake
Computational analysis of Ca2+ dynamics in isolated cardiac mitochondria predicts two distinct modes of Ca2+ uptake
Key points
Cytosolic, but not matrix, Mg2+ inhibits mitochondrial Ca2+ uptake through the Ca2+ uniporter (CU).
The majority of mitochondrial Ca2+ uptake under physiological levels ...
Mechanism of Ca2+Transport by Sarcoplasmic Reticulum
Mechanism of Ca2+Transport by Sarcoplasmic Reticulum
AbstractThe sections in this article are:Structure of Sarcoplasmic Reticulum and Transverse TubulesStructure of Plasmalemma and T TubulesSarcoplasmic ReticulumJunction Between T Tu...
Na+/Ca2+ exchange current in ventricular myocytes of fish heart: contribution to sarcolemmal Ca2+ influx
Na+/Ca2+ exchange current in ventricular myocytes of fish heart: contribution to sarcolemmal Ca2+ influx
ABSTRACT
Influx of extracellular Ca2+ plays a major role in the activation of contraction in fish cardiac cells. The relative contributions of Na+/Ca2+ exchange and ...
A basic model of calcium homeostasis in non-excitable cells
A basic model of calcium homeostasis in non-excitable cells
AbstractThe level of cytosolic calcium (Ca2+) in cells is tightly regulated to about 100 nM (pCa ≈ 7). Due to external stimuli, the basal cytosolic Ca2+level can temporarily be rai...
The emergence of subcellular pacemaker sites for calcium waves and oscillations
The emergence of subcellular pacemaker sites for calcium waves and oscillations
Key points
Calcium (Ca2+) is fundamental to biological cell function, and Ca2+ waves generating oscillatory Ca2+ signals are widely observed in many cell types.
Some experimental s...
Regulation of cochlear hair cell function by intracellular calcium stores
Regulation of cochlear hair cell function by intracellular calcium stores
IntroductionMammalian hearing depends on the dual mechanosensory and motor functions of cochlear hair cells. Both these functions may be regulated by Ca2+ release from intracellula...
British Food Journal Volume 45 Issue 9 1943
British Food Journal Volume 45 Issue 9 1943
I now pass on to an aspect of calcium metabolism which is more topical, but probably more controversial. I refer to the incidence of calcium deficiency. By what means can we determ...