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Choanoflagellate Ciliogenesis Live Imaging v2
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This protocol removes the cilia/flagella from choanoflagellate cells and sets up the cells for live imaging of ciliogenesis. It has been developed for the species Salpingoeca rosetta, but may be portable into other choanoflagellate species. Cells begin to re-generate their cilia/flagella right after removal. The idea of using a cold shock with glycerol for ciliary removal came from Brokaw et al 1960 (doi: 10.1016/0014-4827(60)90027-6).
Title: Choanoflagellate Ciliogenesis Live Imaging v2
Description:
This protocol removes the cilia/flagella from choanoflagellate cells and sets up the cells for live imaging of ciliogenesis.
It has been developed for the species Salpingoeca rosetta, but may be portable into other choanoflagellate species.
Cells begin to re-generate their cilia/flagella right after removal.
The idea of using a cold shock with glycerol for ciliary removal came from Brokaw et al 1960 (doi: 10.
1016/0014-4827(60)90027-6).
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