GW24-e3829 The role of CD59 in mouse cerebral ischaemia-reperfusion injury

Objectives To investigate dynamic changes of complement regulatory protein CD59 expression and discuss its possible mechanisms. During cerebral ischaemia-reperfusion injury. Methods The middle cerebral artery occlusion (MACO) model was induced through filament method. The dynamic changes of neurological deficit scores and brain Pathological injury was recorded. Immunofluorescence and real time RT-PCR method were used to detect complement regulatory protein CD59 expression. Results The time point of the most severest neurological deficit was at 24h after reperfusion, brain pathological injury was the most serious at 24h after reperfusion. The complement regulatory protein CD59 expression decreased gradually after local cerebral ischaemia-reperfusion injury, the lowest at 24h after reperfusion, and returned to normal at 96h after reperfusion. Complement regulatory protein CD59 expression was negative correlated with brain pathological injury and neurological deficit. Conclusions The complement regulatory protein CD59 has a significant change after cerebral ischaemia-reperfusion, it was speculated that participate in the course of the injury after ischaemia-reperfusion. So inhibition of complement by complement regulatory protein CD59 is likely to be a new important method of treatment.