POLARITY GUIDED EXTRACTION AND CHROMATOGRAPHIC IDENTIFICATION OF ACETOGENINS FROM Annona glabra L. LEAVES

Annona glabra L. (family: Annonaceae) is used as a traditional medicine   against several human infirmities, including cancer. Most of the members of   family annonaceae possess a unique class of natural product called   acetogenins. The present study was aimed to extract and fractionate the   acetogenins from the leaves of Annona glabra using the solvents of increasing   polarity, such as hexane, chloroform, ethyl acetate ethanol, and water. Solvents   with higher polarity such as ethyl acetate were found more effective in   extracting lactone-containing compounds as compared to the less polar   solvents. The quantity of acetogenins isolated from the A. glabra leaves was   estimated by lactone concentration test. LC-MS/MS screening showed the   presence of acetogenins in the samples with m/z values 596.9, 606.9, 679.8,   566.8, 550.85, 672.15, 672.15, 624.9 and 565.35. These findings open up   effective methods for separation and quantification of bioactive compounds.  Further studies are to be focused on evaluating the efficacy of these compounds  in pre-clinical and clinical trials, which may potentially contribute in   developing the novel therapeutic formulations.