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Microscopic, histochemical and preliminary phytochemical characterization of leaves of Trema micrantha (L.) Blume

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Para enriquecer el enriquecer el conocimiento sobre Trema micrantha (L.) Blume, esta investigación tuvo como objetivo realizar la caracterización anatómica, histoquímica y fitoquímica de las hojas de la especie. Se realizaron cortes transversales del pecíolo y limbo, así como cortes paradérmicos del limbo, analizados en microscopía óptica y polarizada. Se utilizaron diferentes reactivos para el análisis histoquímico. Se han descrito estructuras anatómicas que proporcionan un diagnóstico detallado de las especies estudiadas. La histoquímica mostró la presencia de metabolitos esenciales (flavonoides, taninos, entre otros) para la especie y mediante análisis SEM-EDS se confirmó que los cristales están compuestos por oxalato de calcio. El análisis fitoquímico permitió la identificación de mono y sesquiterpenos, triterpenos y esteroides, entre otros. El estudio proporcionó datos sin precedentes sobre la especie, ampliando la información científica de T. micrantha. In order to enrich the knowledge about Trema micrantha (L.) Blume, this research aims to perform the anatomical, histochemical and phytochemical characterization of the leaves of this species. Cross sections of the petiole and limbus were made, as well as paradermic sections of the limbus, analyzed in optical and polarized microscopy. Different reagents were used for histochemical analysis. Anatomical structures have been described and provide a detailed diagnosis of the species studied. Histochemistry showed the presence of essential metabolites (flavonoids, tannins, among others) for the species and through SEM-EDS analysis it was confirmed that the crystals are composed of calcium oxalate. Phytochemical analysis allowed the identification of mono and sesquiterpenes, triterpenes and steroids, among others. The study provided unprecedented data on the species, expanding the scientific information of T. micrantha.
Title: Microscopic, histochemical and preliminary phytochemical characterization of leaves of Trema micrantha (L.) Blume
Description:
Para enriquecer el enriquecer el conocimiento sobre Trema micrantha (L.
) Blume, esta investigación tuvo como objetivo realizar la caracterización anatómica, histoquímica y fitoquímica de las hojas de la especie.
Se realizaron cortes transversales del pecíolo y limbo, así como cortes paradérmicos del limbo, analizados en microscopía óptica y polarizada.
Se utilizaron diferentes reactivos para el análisis histoquímico.
Se han descrito estructuras anatómicas que proporcionan un diagnóstico detallado de las especies estudiadas.
La histoquímica mostró la presencia de metabolitos esenciales (flavonoides, taninos, entre otros) para la especie y mediante análisis SEM-EDS se confirmó que los cristales están compuestos por oxalato de calcio.
El análisis fitoquímico permitió la identificación de mono y sesquiterpenos, triterpenos y esteroides, entre otros.
El estudio proporcionó datos sin precedentes sobre la especie, ampliando la información científica de T.
micrantha.
In order to enrich the knowledge about Trema micrantha (L.
) Blume, this research aims to perform the anatomical, histochemical and phytochemical characterization of the leaves of this species.
Cross sections of the petiole and limbus were made, as well as paradermic sections of the limbus, analyzed in optical and polarized microscopy.
Different reagents were used for histochemical analysis.
Anatomical structures have been described and provide a detailed diagnosis of the species studied.
Histochemistry showed the presence of essential metabolites (flavonoids, tannins, among others) for the species and through SEM-EDS analysis it was confirmed that the crystals are composed of calcium oxalate.
Phytochemical analysis allowed the identification of mono and sesquiterpenes, triterpenes and steroids, among others.
The study provided unprecedented data on the species, expanding the scientific information of T.
micrantha.

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