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Label-free quantification in the Crux toolkit
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AbstractUltimately, most tandem mass spectrometry (MS/MS) proteomics experiments aim to not just detect but also quantify the proteins in a given complex sample. Here, we describe an extension to the Crux MS/MS analysis toolkit to enable label-free quantification of peptides. We demonstrate that Crux’s new quantification command, which is modeled after the algorithms implemented in the widely used FlashLFQ software, is both efficient and accurate. In particular, we achieve a 1.9-fold speedup while reducing the memory usage by 26%. The new crux-lfq command is available in Crux v4.3.
Cold Spring Harbor Laboratory
Title: Label-free quantification in the Crux toolkit
Description:
AbstractUltimately, most tandem mass spectrometry (MS/MS) proteomics experiments aim to not just detect but also quantify the proteins in a given complex sample.
Here, we describe an extension to the Crux MS/MS analysis toolkit to enable label-free quantification of peptides.
We demonstrate that Crux’s new quantification command, which is modeled after the algorithms implemented in the widely used FlashLFQ software, is both efficient and accurate.
In particular, we achieve a 1.
9-fold speedup while reducing the memory usage by 26%.
The new crux-lfq command is available in Crux v4.
3.
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