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Quantification of desferrioxamine in blood plasma by inductively coupled plasma atomic emission spectrometry.
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Abstract
A sensitive method, inductively coupled plasma atomic emission spectroscopy, is used to measure desferrioxamine in blood plasma. The desferrioxamine is transformed into its iron chelate, ferrioxamine, which is extracted into benzyl alcohol, then re-extracted into HCl (0.5 mol/L), which is used as the sample for the spectroscopy. For a 0.5-mL plasma sample, the detection limit (1 microgram/mL) suffices for following the concentration of desferrioxamine in plasma after its subcutaneous or intramuscular injection (40 mg per kg of body weight). Neither blood pigments nor trace metals interfere.
Title: Quantification of desferrioxamine in blood plasma by inductively coupled plasma atomic emission spectrometry.
Description:
Abstract
A sensitive method, inductively coupled plasma atomic emission spectroscopy, is used to measure desferrioxamine in blood plasma.
The desferrioxamine is transformed into its iron chelate, ferrioxamine, which is extracted into benzyl alcohol, then re-extracted into HCl (0.
5 mol/L), which is used as the sample for the spectroscopy.
For a 0.
5-mL plasma sample, the detection limit (1 microgram/mL) suffices for following the concentration of desferrioxamine in plasma after its subcutaneous or intramuscular injection (40 mg per kg of body weight).
Neither blood pigments nor trace metals interfere.
Related Results
Studies in Acute Iron Poisoning. I. Desferrioxamine in the Treatment of Acute Iron Poisoning: Clinical Observations, Experimental Studies, and Theoretical Considerations, by Charles F. Whitten et al,Pediatrics, 1965;36:322–335; andStudies in Acute Iron Po
Studies in Acute Iron Poisoning. I. Desferrioxamine in the Treatment of Acute Iron Poisoning: Clinical Observations, Experimental Studies, and Theoretical Considerations, by Charles F. Whitten et al,Pediatrics, 1965;36:322–335; andStudies in Acute Iron Po
Objective. Children with iron poisoning, and mongrel dogs studied under laboratory conditions, were evaluated to explore the safety and effectiveness of the use of desferrioxamine ...
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