In Vitro Studium der Trägerproteine von 3H-markierten juvenilhormonwirksamen Verbindungen in der Hämolymphe von Tenebrio molitor L. Larven / In Vitro Study of the Carrier Proteins of 3H-labelled Juvenile Hormone Active Compounds in the Haemolymph of Teneb

The labelling of Methyl 7,11-dichloro-3,7,11-trimethyl-2-dodecenoate and Methyl 10,11-epoxy-3,7,11-trimethyl-2-cis/trans-dodecenoate with tritium by methylation of the acids with diazomethane in the presence of carrier-free tritium water and the hydrogenation of 10,11-Epoxy-6,7-cis-2,3-trans/cis-farnesyl propynyl ether with titrium gas gave high spec. radioactivities (3.7; 0.49 and 25 Ci/mMol). The strong binding of these hormones to the haemolymph proteins of white newly moulted and of colored larvae was demonstrated by gelfiltration with sephadex G-25. The disc-electrophoretic identification of the carrier proteins in the haemolymph of colored larvae showed that the hormones are approximately total bound by the hormone binding factor of a lipoprotein band. In the haemolymph of white newly moulted larvae there is a second hormone binding band beside the lipoprotein band with similar binding capacity.