Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
A novel type of IRES having variable numbers of eIF4E-binding site and synergistic effect in RNA2 of WYMV isolates
View through CrossRef
AbstractSome viral proteins were translated in cap-independent manner via internal ribosome entry site (IRES), which ever maintained conservative characteristic among different isolates of same species of virus. However, IRES activity presented 7-fold of variance in RNA2 of wheat yellow mosaic virus (WYMV) HC and LYJN isolates. Based on RNA structure probing and mutagenesis assay, the loosened middle stem of H1 and hepta-nucleotide top loop of H2 in LYJN isolate synergistically ensured the higher IRES activity than that in HC isolate. In addition, the conserved top loop of H1 ensured basic IRES activity in HC and LYJN isolates. RNA2 5′-UTR specifically interacted with the wheat eIF4E, which was accomplished by the top loop of H1 in HC isolate or the top loop of H1 and H2 in LYJN isolate. Different IRES activity of WYMV RNA2 was regulated by different numbers of eIF4E-binding site and their synergistic effect, which was accomplished by the proximity of H1 and H2 due to the flexibility of middle stem in H1. It is represented a novel evolution pattern of IRES.
Title: A novel type of IRES having variable numbers of eIF4E-binding site and synergistic effect in RNA2 of WYMV isolates
Description:
AbstractSome viral proteins were translated in cap-independent manner via internal ribosome entry site (IRES), which ever maintained conservative characteristic among different isolates of same species of virus.
However, IRES activity presented 7-fold of variance in RNA2 of wheat yellow mosaic virus (WYMV) HC and LYJN isolates.
Based on RNA structure probing and mutagenesis assay, the loosened middle stem of H1 and hepta-nucleotide top loop of H2 in LYJN isolate synergistically ensured the higher IRES activity than that in HC isolate.
In addition, the conserved top loop of H1 ensured basic IRES activity in HC and LYJN isolates.
RNA2 5′-UTR specifically interacted with the wheat eIF4E, which was accomplished by the top loop of H1 in HC isolate or the top loop of H1 and H2 in LYJN isolate.
Different IRES activity of WYMV RNA2 was regulated by different numbers of eIF4E-binding site and their synergistic effect, which was accomplished by the proximity of H1 and H2 due to the flexibility of middle stem in H1.
It is represented a novel evolution pattern of IRES.
Related Results
Evolution of Antimicrobial Resistance in Community vs. Hospital-Acquired Infections
Evolution of Antimicrobial Resistance in Community vs. Hospital-Acquired Infections
Abstract
Introduction
Hospitals are high-risk environments for infections. Despite the global recognition of these pathogens, few studies compare microorganisms from community-acqu...
Mechanism of Internal Initiation of Translation in Plants
Mechanism of Internal Initiation of Translation in Plants
Original objectives Elucidation of PABP's role in crTMV148 IRES function in-vitro using wheat germ extract and krebs-2 cells extract. Fully achieved. Elucidation of PABP's role in ...
In vitro susceptibility testing of Candida species isolated from blood stream infections to five conventional antifungal drugs
In vitro susceptibility testing of Candida species isolated from blood stream infections to five conventional antifungal drugs
Candida is an opportunistic fungal pathogen which can cause fatal bloodstream infections (BSIs) in immunocompromised and immunodeficient persons. In this study, the susceptibility ...
The regulation of mammalian maternal-to-embryonic transition by Eukaryotic translation initiation factor 4E
The regulation of mammalian maternal-to-embryonic transition by Eukaryotic translation initiation factor 4E
AbstractGenetic and inhibitor studies show expression of eukaryotic translation initiation factor 4E (eIF4E) was required for the successful maternal-to-embryonic transition of mou...
Comparative genomics analyses reveal sequence determinants underlying interspecies variations in injury-responsive enhancers
Comparative genomics analyses reveal sequence determinants underlying interspecies variations in injury-responsive enhancers
Abstract
Background
Injury induces profound transcriptional remodeling events, which could lead to only wound healing, partial tissue repair, or per...
Abstract 1624: eIF4E allosteric regulators cause rapid commitment to apoptosis in cancer cells while sparing immune cells
Abstract 1624: eIF4E allosteric regulators cause rapid commitment to apoptosis in cancer cells while sparing immune cells
Abstract
Advanced or metastatic breast cancers represents a large patient population. Targeted therapies following hormonal or chemotherapeutic approaches offer some...
Data from Translation Initiation Factor eIF4E Is a Target for Tumor Cell Radiosensitization
Data from Translation Initiation Factor eIF4E Is a Target for Tumor Cell Radiosensitization
<div>Abstract<p>A core component in the cellular response to radiation occurs at the level of translational control of gene expression. Because a critical element in tr...
Data from Translation Initiation Factor eIF4E Is a Target for Tumor Cell Radiosensitization
Data from Translation Initiation Factor eIF4E Is a Target for Tumor Cell Radiosensitization
<div>Abstract<p>A core component in the cellular response to radiation occurs at the level of translational control of gene expression. Because a critical element in tr...