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Barcoded Primers: Influenza A Whole Genome Amplification for Nanopore Sequencing v1
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This protocol describes an optimized method for the simultaneous amplification and barcoding of all eight influenza A segments using Barcoded Primers with either the SuperScript IV One-Step RT-PCR Kit (Invitrogen) or the LunaScript Multiplex One-Step RT-PCR Kit (NEB). The purified barcoded amplicons can be used for Oxford Nanopore library preparation with the Ligation Sequencing Kit for multiplexed whole-genome sequencing of influenza A virus genomes.
Title: Barcoded Primers: Influenza A Whole Genome Amplification for Nanopore Sequencing v1
Description:
This protocol describes an optimized method for the simultaneous amplification and barcoding of all eight influenza A segments using Barcoded Primers with either the SuperScript IV One-Step RT-PCR Kit (Invitrogen) or the LunaScript Multiplex One-Step RT-PCR Kit (NEB).
The purified barcoded amplicons can be used for Oxford Nanopore library preparation with the Ligation Sequencing Kit for multiplexed whole-genome sequencing of influenza A virus genomes.
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