Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Barcoded Primers: Influenza A Whole Genome Amplification for Nanopore Sequencing v1
View through CrossRef
This protocol describes an optimized method for the simultaneous amplification and barcoding of all eight influenza A segments using Barcoded Primers with either the SuperScript IV One-Step RT-PCR Kit (Invitrogen) or the LunaScript Multiplex One-Step RT-PCR Kit (NEB). The purified barcoded amplicons can be used for Oxford Nanopore library preparation with the Ligation Sequencing Kit for multiplexed whole-genome sequencing of influenza A virus genomes.
Title: Barcoded Primers: Influenza A Whole Genome Amplification for Nanopore Sequencing v1
Description:
This protocol describes an optimized method for the simultaneous amplification and barcoding of all eight influenza A segments using Barcoded Primers with either the SuperScript IV One-Step RT-PCR Kit (Invitrogen) or the LunaScript Multiplex One-Step RT-PCR Kit (NEB).
The purified barcoded amplicons can be used for Oxford Nanopore library preparation with the Ligation Sequencing Kit for multiplexed whole-genome sequencing of influenza A virus genomes.
Related Results
Custom barcoded primers for influenza A nanopore sequencing: enhanced performance with reduced preparation time
Custom barcoded primers for influenza A nanopore sequencing: enhanced performance with reduced preparation time
Highly pathogenic avian influenza is endemic and widespread in wild birds and is causing major outbreaks in poultry worldwide and in U.S. dairy cows, with several recent human case...
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
Human tissues comprise trillions of cells that populate a complex space of molecular phenotypes and functions and that vary in abundance by 4–9 orders of magnitude. Relying solely ...
U.S. utilization patterns of influenza antiviral medications during the 2009 H1N1 influenza pandemic
U.S. utilization patterns of influenza antiviral medications during the 2009 H1N1 influenza pandemic
Please cite this paper as: Borders‐Hemphill and Mosholder (2012) U.S. utilization patterns of influenza antiviral medications during the 2009 H1N1 influenza pandemic. Influenza and...
Pipeline for species-resolved full-length16S rRNA amplicon nanopore sequencing analysis of low-complexity bacterial microbiota
Pipeline for species-resolved full-length16S rRNA amplicon nanopore sequencing analysis of low-complexity bacterial microbiota
Abstract
16S rRNA amplicon sequencing is a fundamental tool for characterizing prokaryotic microbial communities. While short-read 16S rRNA sequencing is a proven s...
Next Generation Sequencing Technologies and Their Applications
Next Generation Sequencing Technologies and Their Applications
Abstract
The advances in next generation sequencing (NGS) technologies have tremendous impacts on the studies of structural and f...
238. Direct identification of Bacterial Species with MinION Nanopore Sequencer In Clinical Specimens Suspected of Polybacterial Infection
238. Direct identification of Bacterial Species with MinION Nanopore Sequencer In Clinical Specimens Suspected of Polybacterial Infection
Abstract
Background
Conventional culture tests usually identify only a few bacterial species, which can grow well in the culture...
A severe 2017 influenza season dominated by influenza A(H3N2), Victoria, Australia
A severe 2017 influenza season dominated by influenza A(H3N2), Victoria, Australia
Surveillance for influenza-like illness (ILI) and laboratory-confirmed influenza in Victoria, Australia is undertaken jointly by the Victorian Infectious Diseases Reference Laborat...
Whole Genome Resequencing and 1000 Genomes Project
Whole Genome Resequencing and 1000 Genomes Project
Abstract
The recent advances in sequencing technologies have enabled the whole human genome to be sequenced within weeks. To date, several human...