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Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
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ABSTRACT
An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the
Escherichia coli
genome by using the
Streptococcus pyogenes
type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in
Tatumella citrea
, another species of the
Enterobacteriaceae
, with highest efficiency of 100%.
American Society for Microbiology
Title: Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Description:
ABSTRACT
An efficient genome-scale editing tool is required for construction of industrially useful microbes.
We describe a targeted, continual multigene editing strategy that was applied to the
Escherichia coli
genome by using the
Streptococcus pyogenes
type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets.
The system also demonstrated successful targeted chromosomal deletions in
Tatumella citrea
, another species of the
Enterobacteriaceae
, with highest efficiency of 100%.
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