Antibacterial mechanism of inosine againstAlicyclobacillus acidoterrestris

AbstractInosine could potentially become a novel antibacterial agent againstA. acidoterrestrisas low-dose of inosine can preventA. acidoterrestriscontamination. However, until now the antibacterial mechanism of inosine targetingA. acidoterrestrisis still unknown. In this study, to unravel the mechanism of inosine againstA. acidoterrestrispuzzle, the effects of inosine on bacterial surface hydrophobicity, intracellular protein content, cell membrane damage extent and permeability of theA. acidoterrestriswere investigated. The results showed that inosine can effectively inhibit the growth and reproduction ofA. acidoterrestrisby destroying the integrity of cell membrane and increasing its permeability, causing the leakage of intracellular nutrients. Furthermore, the interaction networks of inosine target proteins were analyzed. The interaction networks further revealed that damage of bacterial cell membrane might be relevant to inosine’s effect on bacterial DNA replication and cell energy metabolism through regulating nucleotide synthesis and metabolism and the activity of translation initiation factors. Finally, the antibacterial mechanism of inosine againstA. acidoterrestriswas proposed.