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Bacterial genome annotation script using BLASTN v1
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This protocol uses a python based script and command-line blastn to annotate Sanger sequencing results from genome amplifications. Its main use in our lab (https://biocomputationlab.com) is to identify the location and gene locus of transposon inserts in microbial bacterial genomes of Pseudomonas putida KT2440. However, this script can be used for other bacterial genomes for which its genome sequence and annotation are available. Script was developed in python 3.9 with blastn version 2.2.18.
Title: Bacterial genome annotation script using BLASTN v1
Description:
This protocol uses a python based script and command-line blastn to annotate Sanger sequencing results from genome amplifications.
Its main use in our lab (https://biocomputationlab.
com) is to identify the location and gene locus of transposon inserts in microbial bacterial genomes of Pseudomonas putida KT2440.
However, this script can be used for other bacterial genomes for which its genome sequence and annotation are available.
Script was developed in python 3.
9 with blastn version 2.
2.
18.
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