Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Cloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B
View through CrossRef
Polysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses. Therefore, heterologous production of PMOs is required for the development of their application. We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production.Here we cloned the NCU08746 gene, an AA13 PMO from Neurospora crassa, into pEX2B. We successfully designed primers and amplified the NCU08746 gene from the N. crassa genome. NCU08746 was then cloned into the pEX2B vector. The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A. tumefaciens AGL1 prior to being transferred into A. oryzae. This work provides the foundation for further study on PMOs expression in A. oryzae.
Truong Dai hoc Nguyen Tat Thanh
Title: Cloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B
Description:
Polysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses.
Therefore, heterologous production of PMOs is required for the development of their application.
We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production.
Here we cloned the NCU08746 gene, an AA13 PMO from Neurospora crassa, into pEX2B.
We successfully designed primers and amplified the NCU08746 gene from the N.
crassa genome.
NCU08746 was then cloned into the pEX2B vector.
The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A.
tumefaciens AGL1 prior to being transferred into A.
oryzae.
This work provides the foundation for further study on PMOs expression in A.
oryzae.
Related Results
DFT studies of the copper active site in AA13 polysaccharide monooxygenase
DFT studies of the copper active site in AA13 polysaccharide monooxygenase
AA13 polysaccharide monooxygenases (AA13 PMOs) are novel enzymes that break down starch using a copper active site in a substrate binding groove on a solvent-exposed surface. The s...
Plasmid-encoded regulation of colicin E1 gene expression
Plasmid-encoded regulation of colicin E1 gene expression
A plasmid-encoded factor that regulates the expression of the colicin E1 gene was found in molecular cloning experiments. The 2,294-base-pair AvaII fragment of the colicin E1 plasm...
Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers
Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers
Ammonia oxidizers (family Nitrobacteraceae) and methanotrophs (family Methylococcaceae) oxidize CO and CH4 to CO2 and NH4+ to NO2-. However, the relative contributions of the two g...
Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost
Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost
ABSTRACTPlasmids play a major role in rapid adaptation of bacteria by facilitating horizontal transfer of diverse genes, most notably those conferring antibiotic resistance. While ...
Role of Phosphorylation in Fungal Spore Germination
Role of Phosphorylation in Fungal Spore Germination
Spore germination is a common and fundamental event in fungal development and in many instances an essential phase of fungal infection and dissemination. Spore germination is also ...
Expression and polymorphism of genes in gallstones
Expression and polymorphism of genes in gallstones
ABSTRACT
Through the method of clinical case control study, to explore the expression and genetic polymorphism of KLF14 gene (rs4731702 and rs972283) and SR-B1 gene (rs...
ENHANCING YIELD OF LOW COPY NUMBER PLANT BASED VECTOR FROM E.COLI CELLS FOR GENE CLONING
ENHANCING YIELD OF LOW COPY NUMBER PLANT BASED VECTOR FROM E.COLI CELLS FOR GENE CLONING
Plasmid DNA is an important role play for gene cloning and gene expression analysis in E.coli cells. But some of the plasmids are very low copy number so its isolation is also diff...
A new device-mediated miniprep method
A new device-mediated miniprep method
AbstractSmall-scale plasmid DNA preparation or miniprep is a fundamental technique in estimation cloning experiments and is widely used for DNA methylation analysis in epigenetic r...