Abstract 3333: Cancer stem cell-mediated regulation of Myc overexpressing medulloblastoma cells on proliferation, migration and invasion

Abstract Medulloblastoma (MB) is the most common and malignant pediatric brain tumor. Myc amplification was frequently shown in the tumor. Hereby cancer stem cells are being issued lately was investigated whether it is associated with Myc and its contributions. MB cell lines - UW228, UW228-Myc, UW426 and UW426-Myc were used for following assays. Myc overexpressing cells of two kinds of cells were shown significantly higher proliferation rate than counterpart cells by cell proliferation assay. In particular, the extent of difference in UW228-Myc was bigger than in UW426-Myc. Using cell cycle analysis, DNA contents in both UW228-Myc and UW426-Myc were mainly accumulated in G2/M phase compared with their each control, suggesting that cells overexpressing Myc have faster proliferation rate. In addition, two UW228-Myc and UW426-Myc cells were demonstrated more increased self-renewal capacities by colony-forming assay versus UW228 and UW426, respectively. For migration and invasiveness, both Myc overexpressing cells increased in comparison with each control by wound-healing assay and invasion assay using Matrigel chamber. Interestingly UW228-Myc cells were shown nearly six times higher invasiveness than in UW228 cells. In contrast, the disparity of that in UW426 and UW426-Myc cells was just about two times. It is verified that why Myc overexpressing cells have more dominant proliferation, self-renewal, migration and invasion abilities than corresponding controls by western blot assay. The results showed strongly increased expression of migration, invasion-related markers (p-AKT, p-ERK1/2 and Rac1) and cancer stem cell-related markers (Nestin, CD133, Oct3/4 and SOX2), especially in UW228-Myc cells. In conclusion, Myc expression induces not only cell proliferative and self-renew propensities, but also migration and invasiveness by mediating cancer stem cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3333. doi:1538-7445.AM2012-3333