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Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids
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The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.
American Association of Swine Veterinarians
Title: Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids
Description:
The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms.
Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative.
Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs.
At S:P ≥ 0.
40, diagnostic sensitivity and specificity were estimated as 97.
6% and 100.
0%, respectively.
Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.
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