Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Transcriptional Regulation and PosttranslationalActivity of the Betaine Transporter BetL in Listeriamonocytogenes Are Controlled by EnvironmentalSalinity
View through CrossRef
ABSTRACT
While
the genetic elements contributing to the salinity tolerance of
Listeria monocytogenes
have been well characterized, the
regulatory signals and responses (genetic and/or biochemical) that
govern these mechanisms have yet to be elucidated. Encoded by
betL
, the first genetic element to be linked to listerial
osmotolerance, the secondary betaine uptake system BetL is a member of
the betaine-carnitine-choline transporter family. Preceded by consensusσ
A
- and σ
B
-dependent promoter
sites,
betL
is constitutively expressed and transcriptionally
up-regulated in response to salt stress. The nisin-controlled
expression system was used to achieve salinity-independent, controlled
betL
expression in
Listeria
. In the absence of
NaCl-activated transcriptional control, BetL activity was found to be a
function of environmental salinity, showing optimal activity in buffer
supplemented with 1 to 2% NaCl (osmolality, 417 to 719
mosmol/kg). In addition, BetL was activated rapidly (half-life, 2 min)
in response to an osmotic upshift imposed by adding 2% NaCl to
50 mM potassium phosphate
buffer.
American Society for Microbiology
Title: Transcriptional Regulation and PosttranslationalActivity of the Betaine Transporter BetL in
Listeriamonocytogenes
Are Controlled by EnvironmentalSalinity
Description:
ABSTRACT
While
the genetic elements contributing to the salinity tolerance of
Listeria monocytogenes
have been well characterized, the
regulatory signals and responses (genetic and/or biochemical) that
govern these mechanisms have yet to be elucidated.
Encoded by
betL
, the first genetic element to be linked to listerial
osmotolerance, the secondary betaine uptake system BetL is a member of
the betaine-carnitine-choline transporter family.
Preceded by consensusσ
A
- and σ
B
-dependent promoter
sites,
betL
is constitutively expressed and transcriptionally
up-regulated in response to salt stress.
The nisin-controlled
expression system was used to achieve salinity-independent, controlled
betL
expression in
Listeria
.
In the absence of
NaCl-activated transcriptional control, BetL activity was found to be a
function of environmental salinity, showing optimal activity in buffer
supplemented with 1 to 2% NaCl (osmolality, 417 to 719
mosmol/kg).
In addition, BetL was activated rapidly (half-life, 2 min)
in response to an osmotic upshift imposed by adding 2% NaCl to
50 mM potassium phosphate
buffer.
Related Results
Identification and Disruption of BetL, a Secondary Glycine Betaine Transport System Linked to the Salt Tolerance of
Listeria monocytogenes
LO28
Identification and Disruption of BetL, a Secondary Glycine Betaine Transport System Linked to the Salt Tolerance of
Listeria monocytogenes
LO28
ABSTRACT
The trimethylammonium compound glycine betaine (
N
,
N
,
N
-trimethylglycine...
Molecular and Physiological Analysis of the Role of Osmolyte Transporters BetL, Gbu, and OpuC in Growth of
Listeria monocytogenes
at Low Temperatures
Molecular and Physiological Analysis of the Role of Osmolyte Transporters BetL, Gbu, and OpuC in Growth of
Listeria monocytogenes
at Low Temperatures
ABSTRACT
Listeria monocytogenes
is a ubiquitous food-borne pathogen found widely distributed in nature as well as an undesirable contaminant in a...
Accumulation of glycine betaine and partial purification of betaine aldehyde dehydrogenase from a halotolerant cyanobacterium aphanothece halophytica
Accumulation of glycine betaine and partial purification of betaine aldehyde dehydrogenase from a halotolerant cyanobacterium aphanothece halophytica
The H1-NMR spectroscopy was a suitable method for the determination of glycine betaine in a halotolerant cyanobacterium, A.halophytica. The amount of glycine betaine from A.halophy...
Multiple Deletions of the Osmolyte Transporters BetL, Gbu, and OpuC of
Listeria monocytogenes
Affect Virulence and Growth at High Osmolarity
Multiple Deletions of the Osmolyte Transporters BetL, Gbu, and OpuC of
Listeria monocytogenes
Affect Virulence and Growth at High Osmolarity
ABSTRACT
The success of
Listeria monocytogenes
as a food-borne pathogen owes much to its ability to survive a variety of stresses, bo...
Natural liquid betaine dietary supplementation improves growth performance, immuno-antioxidant responses, and stress resistance in Nile tilapia subjected to acute ammonia challenge
Natural liquid betaine dietary supplementation improves growth performance, immuno-antioxidant responses, and stress resistance in Nile tilapia subjected to acute ammonia challenge
Abstract
This work investigated the effects of natural liquid betaine (NATURA BETAINE) dietary supplementation on physiological performance a...
Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone
Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone
Osteoarthritis (OA) is the most common type of arthritis with no effective therapy. Subchondral bone and overlying articular cartilage are closely associated and function as “osteo...
Acceleration of liver regeneration by betaine supplementation via enhancement of sulfur‐containing amino acid metabolism
Acceleration of liver regeneration by betaine supplementation via enhancement of sulfur‐containing amino acid metabolism
Our earlier work showed that hepatic S‐adenosylmethionine (SAM) level was critically associated with liver regenerative process. In this study we examined the progression of liver ...
Compatibility of glycinebetaine in rice plants: evaluation using transgenic rice plants with a gene for peroxisomal betaine aldehyde dehydrogenase from barley
Compatibility of glycinebetaine in rice plants: evaluation using transgenic rice plants with a gene for peroxisomal betaine aldehyde dehydrogenase from barley
ABSTRACTGlycinebetaine is synthesized in plants by the two‐step oxidation of choline, with betaine aldehyde as the intermediate. The reactions are catalyzed by choline mono‐oxygena...