Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Evaluation of Slowfade Diamond as a buffer for STORM microscopy
View through CrossRef
We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568. Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.
Optica Publishing Group
Title: Evaluation of Slowfade Diamond as a buffer for STORM microscopy
Description:
We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy.
We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568.
Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.
Related Results
Penanaman Nilai-Nilai Islam dalam Konstruksi Kemuliaan Intan oleh Pedagang Intan untuk Memelihara Stabilitas Pasar Intan di Martapura Kalimantan Selatan
Penanaman Nilai-Nilai Islam dalam Konstruksi Kemuliaan Intan oleh Pedagang Intan untuk Memelihara Stabilitas Pasar Intan di Martapura Kalimantan Selatan
The glory of diamond as eternal and scarce thing and the symbol of love is a construction made by diamond merchants to stabilize diamond market. For diamond merchants in Martapura,...
Effects of annealing time on the microstructural and electrochemical properties of B-doped nanocrystalline diamond films
Effects of annealing time on the microstructural and electrochemical properties of B-doped nanocrystalline diamond films
The effects of annealing time under 1000 ℃ on the microstructural and the electrochemical properties of boron-doped nanocrystalline diamond (BDND) films are investigated by HRTEM, ...
Sedimentary characteristics and model of Cambrian storm in Xuzhou area
Sedimentary characteristics and model of Cambrian storm in Xuzhou area
AbstractThe storm deposition types within the Dabeiwang area of Xuzhou City, Jiangsu Province, China, showcase a wide range of diversity and distinctly apparent characteristics. Ba...
Dynamics of dust storm and its response to meteorological conditions and anthropogenic impact in South edge of Taklimakan desert, China
Dynamics of dust storm and its response to meteorological conditions and anthropogenic impact in South edge of Taklimakan desert, China
In this study, the varying trends of dust storm frequency in a typical oasis located at the South edge of Taklimakan desert, China were analyzed by using time series analysis and r...
Heterogeneous interfacial chemical nature and bonds in an
Al
matrix composite reinforced with
W
‐coated diamond particles
Heterogeneous interfacial chemical nature and bonds in an
Al
matrix composite reinforced with
W
‐coated diamond particles
Over the last decade, a diamond particle reinforced Al matrix, namely diamond/Al, composite has been developed for thermal management applications, mainly in the microelectronic in...
Allure of the Abroad: Tiffany & Co., Its Cultural Influence, and Consumers
Allure of the Abroad: Tiffany & Co., Its Cultural Influence, and Consumers
Introduction Tiffany and Co. is an American luxury jewellery and specialty retailer with its headquarters in New York City. Each piece of jewellery, symbolically packaged in a blue...
Procedure for Western blot v1
Procedure for Western blot v1
Goal: This document has the objective of standardizing the protocol for Western blot. This technique allows the detection of specific proteins separated on polyacrylamide gel and t...
Meteorological and Remote Sensing Analysis of the Severe Storm “Daniel” over Greece
Meteorological and Remote Sensing Analysis of the Severe Storm “Daniel” over Greece
Omega blocking, a meteorological phenomenon characterized by a persistent high-pressure system resembling the Greek letter omega (Ω) in the atmosphere, has recently been o...