Abstract A08: A novel lncRNA RGMB-AS inhibit NSCLC metastasis via upregulating the expression of target gene RGMB

Abstract Background: The relationships between long noncoding RNA (lncRNAs) and tumors have currently become one of the focuses on cancer studies. Our previous studies found repulsive guidance molecule b (RGMB) was downregulated in lung cancer which is associated with poor prognosis and cancer metastasis.The present study investigated the regulation of RGMB expression by lncRNA RGMB-AS and its role in lung cancer progression. Methods: The expression of lncRNA RGMB-AS and RGMB were detected in 56 paired NSCLC tissues and adjacent normal tissues by qRT-PCR assay. The relations of lncRNA RGMB-AS and RGMB expression with clinicopathological factors of NSCLC patients were explored. The lncRNA RGMB-AS was downregulated and overexpressed in A549 and H1299 cells, and its role in lung cancer was further investigated in vitro and in vivo. Results: The results of qRT-PCR showed that lncRNA RGMB-AS expression was significantly downregulated in NSCLC tissues than in adjacent normal tissues (P < 0.05). Correlation analysis indicated that the expression of lncRNA RGMB-AS and RGMB mRNA were positively correlated (P < 0.05). The downregulation of lncRNA RGMB-AS in NSCLC tissues were associated with poor overall survival status(P < 0.05). Downregulation of lncRNA RGMB-AS in A549 and H1299 cells subsequently results in reduced RGMB mRNA and protein expression. The downregulation/overexpression of lncRNA RGMB-AS significantly promoted/inhibited NSCLC cells metastasis in vitro and in vivo. Conclusion: We identified lncRNA RGMB-AS which was downregulated in NSCLC patients and thereby to reduce RGMB expression. lncRNA RGMB-AS might inhibit lung cancer metastasis via the regulation on RGMB and other tumor suppressors. Citation Format: Jin Li, Shi Xiaoshun, He Jianxing. A novel lncRNA RGMB-AS inhibit NSCLC metastasis via upregulating the expression of target gene RGMB. [abstract]. In: Proceedings of the AACR Special Conference on Noncoding RNAs and Cancer: Mechanisms to Medicines ; 2015 Dec 4-7; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2016;76(6 Suppl):Abstract nr A08.