Validation Efficiency of Radiation on Serratia Marcescens

Background: Serratia marcescens is a pathogen bacterium (gram negative) that has a rod shape; it is considered as high risk bacteria as antibiotic resistance, and a lot of studies were carried out to limit S. marcescens activity using radiation. Objective: This research aimed to determine the activity of S. marcescens against certain antibiotic, and the radiation affect on this bacterium. Materials and Methods: 25 of diagnosed S. marcescens cross-sectional study design, were collected from Baghdad hospitals from different patients, the patients were vestige to radiation by cultivated on media at 37°C to reach the culture of the stationary-phase, then centrifugation (for 10 minutes at 5000 rpm), and suspended in saline. Then exposed to diverse types of radiations (using only one millimeter), the collected data then compared with group that was not exposed to radiation (control group), rediluted and inoculated in media, cultivated within test tube containing distilled water 5ml, then exposing to an different radiosources including CS137 and CO60 with activity 1-10 μci at different radiation doses for one hour. Results: The results exhibition of gamma, beta rays emitted by CO60 with activity 1 μci on S. marscences inclusive D (1hrs.)=1.55599*10-5, D (2hrs.)=3.111989*10-5, D (3hrs.)=4.86798*10-5 were viable cell in 1hr and compared with control=196. The results of exhibition of Gamm, Beta rays emitted by CO60 with activity 10 μci on S. marcescens inclusive D(1hrs.)= 2.3323* 10-5, D (2hrs.)= 4.6647*10-5, D (3hrs.)= 6.99705*10-5 were viable cell in 1hr, and compared with control=196. Same measurement was carried out for gamma, Beta rays emitted by Cs137 with activity 1 μci and 10 μci and compared with control=196. Statistical Analysis System-SAS program all results are significant. Conclusions: S. marcescens shows high resistance as a multidrug resistance, radiation emitted from semiconductor laser have high efficiency for killing S. marcescens.