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Genotyping of rotaviruses isolated from sewage
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Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4. The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.
American Society for Microbiology
Title: Genotyping of rotaviruses isolated from sewage
Description:
Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4.
The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.
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