Identification and HPLC Purification of Aspirin (Acetylsalicylic Acid) from the Seed Coats, Leaves and Bark of Givotia Rottleriformis Griff.

Abstract Aspirin (Acetylsalicylic acid; ASA) is an anti-inflammatory and anti-cancer molecule derived from salicylic acid (SA) and produced by different companies worldwide because of the wide range of therapeutic applications in human. Accumulated evidence suggest that ASA relieves pain, swelling, cold or flu, prevent colorectal cancer and cardiovascular diseases. ASA induces defense response against wide range of pathogens in plants. Recently, we reported benzoylsalicylic acid (BzSA) and other anti-cancer molecules such as gallic acid (GA) methyl gallate from the seed coats of G. rottleriformis 1-3. Here we are reporting the natural aspirin first time from the seed coats, leaves and bark of G. rottleriformis. HPLC chromatogram of hexane extract of seed coats, leaves and bark have shown a peak at RT 24.8min coeluted with the aspirin standard. The purified aspirin from the seed coats were subjected to 1H NMR and were confirmed as aspirin. These results suggest that aspirin biosynthesis is taking place in seed coats, leaves and bark of G. rottlerifirmis and supports the medicinal properties of G. rottleriformis. These results suggest that the seedcoats, leaves and bark of this plant contains the highly useful medicinal compounds to treat the rheumatism, cancer, cardiovascular, psoriasis, anti-inflammatory and other skin diseases.