ANTIOXIDANT ACTIVITY OF FOREST MANGGOSTEEN (Garcinia hombroniana Pierre) FRACTION USING DPPH AND ABTS METHOD

This study was carried out to determine the active antioxidant fraction of Garcinia hombroniana bark extract by the DPPH and ABTS method. Along with this, the study also attempts to identify the class of compounds present in the various extract of G. hombroniana by the active fraction. The bark extract of G. hombroniana was prepared by the multilevel maceration method by using three solvents including n-hexane, ethyl acetate, and 96% ethanol. Results of study suggested that n-hexane (HSE), ethyl acetate (EASE) and ethanol 96% extract (ESE) have antioxidant activity with IC50 value of 423 ± 16.72 μg/mL, 284.89 ± 2.7μg/mL, and 10.49 ± 0.19 μg/mL in DPPH assay, while these extracts showed IC50 value of 560.92 ± 48.86 μg/mL, 430.18 ± 16.65 μg/mL, and 13.92 ± 0.57 μg/mL respectively in ABTS assay. Further, fractionated using vacuum column chromatography revealed the presence of five fractions viz., A, B, C, D, and E. Among these, fractions D showed the highest antioxidant activity with an IC50 value of 4.83 ± 0.18 μg/mL and 6.82 ± 0.31 μg/mL in DPPH and ABTS assays. Results of the fractioned analysis and qualitative determination showed that the active fraction of G. hombroniana contained flavonoid, triterpenoid, alkaloid, and saponin compounds, and antioxidant activities of these extracts might be due to the presence of these active ingredients.