The Effects Of N-Acetylcysteine on Asprosine Immunoreactivity in A Rat Ovarian Torsion-Detorsion Model

Abstract Objective: Ovarian torsion is a life-threatening gynecological emergency that often necessitates surgical intervention. This study aimed to evaluate the effects of N-acetylcysteine (NAC), a potent antioxidant, on ovarian reserve and asprosin levels in a rat model of torsion-detorsion. Materials and Methods: Thirty-five Wistar albino rats were randomly assigned to five groups: Group I (Control), Group II (Sham), Group III (NAC), Group IV (Torsion/Detorsion - T/D), and Group V (Torsion/Detorsion + NAC). Ovarian and blood samples were collected to assess asprosin immunoreactivity in tissue, serum asprosin levels, anti-Müllerian hormone (AMH) concentrations, and total oxidant status (TOS). Histopathological changes in ovarian tissues were evaluated using immunohistochemical techniques. Results: Serum TOS levels were significantly elevated in the T/D group (p = 0.016) compared to the control group, and significantly decreased in the T/D + NAC group (p = 0.032) compared to the T/D group. Serum asprosin levels were significantly lower in the NAC (p = 0.002), T/D (p = 0.003), and T/D + NAC (p = 0.010) groups compared to the control (overall p = 0.016). However, asprosin levels were significantly higher in the T/D + NAC group than in the T/D group (p = 0.008). In ovarian tissue, asprosin immunoreactivity was significantly reduced in the T/D group compared to the control (p = 0.002), but increased in the T/D + NAC group compared to the T/D group (p = 0.002). No statistically significant differences were observed in AMH levels among the groups (p > 0.05). Conclusion: N-acetylcysteine (NAC) effectively reduced oxidative stress and prevented degenerative changes associated with ovarian torsion-detorsion injury. Asprosin levels appeared to reflect ischemia-reperfusion injury, decreasing with torsion and partially recovering with NAC treatment.