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Advances in hybridoma preparation using electrofusion technology
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As a rapidly developing cell engineering technique, cell electrofusion
has been increasingly applied in the field of hybridoma preparation in
recent years. However, electrofusion is a certain degree of difficulty
to completely replace the polyethylene glycol-mediated cell fusion. The
key elements limiting electrofusion in the field of hybridoma
preparation are practical complicated. This review summarizes the state
of art of cell electrofusion in hybridoma preparation based on recent
published literatures, mainly focusing on electrofusion instruments and
their components, process control, cell treatment, and process
characterization. The review provides new information and insightful
commentary, critically important to the promotion of further
electrofusion development in the field of hybridoma preparation.
Title: Advances in hybridoma preparation using electrofusion technology
Description:
As a rapidly developing cell engineering technique, cell electrofusion
has been increasingly applied in the field of hybridoma preparation in
recent years.
However, electrofusion is a certain degree of difficulty
to completely replace the polyethylene glycol-mediated cell fusion.
The
key elements limiting electrofusion in the field of hybridoma
preparation are practical complicated.
This review summarizes the state
of art of cell electrofusion in hybridoma preparation based on recent
published literatures, mainly focusing on electrofusion instruments and
their components, process control, cell treatment, and process
characterization.
The review provides new information and insightful
commentary, critically important to the promotion of further
electrofusion development in the field of hybridoma preparation.
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