DETERMINATION THE CONCENTRATION OF ASIATICOSIDE IN CENTELLA ASIATICA (L.) URB. – APIACEAE AT QUANG DIEN DISTRICT, THUA THIEN HUE PROVINCE

Background: Centella asiatica (L.) Urb. - Apiaceae has been used in traditional medicine to cure many diseases such as cholera dysentery, pimples, discharge, cough, sore throat, hemorrhoids and detoxification. The objective is to determine the amount of active ingredient asiaticoside in Centella asiatica (L.) Urb at Quang Tho 2 cooperatives, Quang Dien District, Thua Thien Hue province. Materials and method: C.asiatica after being harvested, washed and drained was dried in sunlight or in dryer with different temperature. Each 20g of C.asiatica was extracted using various methods with dissolving appropriate solvents. The extracted solution was diluted by extracted solvent. This diluted solution was filtered through 0.45μm membrane filter for the purpose of chromatography. Results: The chromatography condition: mobile phase consisting of methanol: Trifluoracetic acid 1% (60: 40); Column: HiQ Sil C18 (4.6 x 250 mmm) 3μm; flow rate at 0.7 ml/min; The maximum detection wavelength: 215 nm; retention time of asiaticosid (tR): 18.7 minutes. Conclusion: The content of asiaticoside in sample is from 0.09% to 1.35% depending on the initial sample handling and sample extraction method. Keywords: Centella asiatica (L.) Urb. - Apiaceae, asiaticoside, HPLC.